Fig. 3

Enhanced immunodepletion of AD seeds by affinity improved dmCBTAU-22.1. Residual seeding activity of human AD brain homogenates following immunodepletion with different concentrations of CBTAU-22.1 (blue) and dmCBTAU-22.1 (red) as measured by FRET signal in biosensor cells expressing the microtubule repeat domains of tau (aa 243–375) fused either to yellow or cyan fluorescent protein. Uptake of exogenous tau aggregates into the cells results in aggregation of the tau fusion proteins, which is detected by FRET. As positive and negative controls, a human IgG1 chimeric version of murine anti-PHF antibody AT8 (green) and anti-RSV-G antibody CBRSV-4.1 (black) were taken along, respectively. Error bars indicate the SD of two independent experiments