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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Sporadic Parkinson’s disease derived neuronal cells show disease-specific mRNA and small RNA signatures with abundant deregulation of piRNAs

Fig. 1

mRNA-based quality controls (a) Level plot of all correlation values of rlog normalized gene expression counts. Fibroblasts, iPSCs and neuronal cells show distinct gene expression patterns on a global scale. b Principal component analysis of fibroblasts, iPSCs/ESCs and neurons together with dopaminergic neurons from a literature cohort. The neurons generated cluster well with dopaminergic neurons available in databases [34]. c PluriTest [40] adaption [57] showing high similarity between embryonic stem cells and induced pluripotent stem cells in our cohort. d CTRL and PD iPSCs are comparable in their viral transgene silencing as well as in the range of a published iPSC cohort [1]. Human embryonic stem cells serve as a negative control. Only cells from databases were analysed that passed the pluripotency assessment. The red line depicts the statistical upper-limit cut-off calculated as described in [57]. e Expression analysis of published markers of insufficient viral transgene silencing. No cell line shows a consistent upregulation of markers of insufficient viral transgene silencing. The red line depicts the upper-limit cut-off calculated as described in [57]

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