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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Dipeptide repeat proteins activate a heat shock response found in C9ORF72-ALS/FTLD patients

Fig. 3

DPRs induce expression of C9ORF72 signature transcripts in human neurons. a Diagram of generation of human neurons from stem cells. b Viability dose response curve of human stem cells and stem-cell derived neurons exposed to various DPRs (n = 6). c, d qRT-PCR of C9ORF72-chaperome transcripts (c) and HSF1 (d) in human stem cell-derived motor neurons following treatment with DPRs (poly-GA, poly-GP, poly-GR) or a scrambled poly-GAPR (5 uM for 24 h) normalized to control (DMSO-treated) neurons (mean ± SD, n = 3, one-way ANOVA with Dunnett’s post-hoc test for upregulated genes in DPR-treated neurons compared to control, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

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