Fig. 6

Effects of eEF2K inhibition on mitochondrial dysfunction and oxidative stress induced by human AS in differentiated N2A cells. a-b Measurements of basal oxygen consumption rate-OCR (b) and ATP levels (c) in N2A cells subsequent to transient overexpression of human wild type or mutant A53T AS, with or without siRNA mediated eEF2K knockdown (n = 9–12/group from three independent experiments; Unpaired T-test, *p < 0.05, **p < 0.01, ***p < 0.005; error bars indicate Mean ± S.D.). c Flow cytometry analysis of reactive oxygen species (ROS), measured by DCFDA staining, in N2A cells subsequent to transient overexpression of human wild type or mutant A53T AS, with or without siRNA mediated eEF2K knockdown (n = 9/group from three independent experiments; Unpaired T-test, *p < 0.05, **p < 0.01, ***p < 0.005; error bars indicate Mean ± S.D.)