Fig. 2
From: Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma
DIPG and aGBM demonstrate differential CD3+ lymphocyte infiltration (a-c) Immunohistochemical staining for CD3 (red) of primary DIPG (a), adult GBM (b), and pediatric cerebral cortex (c) tissue, counterstained with hematoxylin (blue). Scale bar = 50 μm (d-e) FACS plots showing different CD3+ composition of CD45+ cells in DIPG (d) and aGBM (e). Samples were gated for size, singularity, viability, and CD45 positivity prior to these plots (f) Table of lymphocyte (CD3+) fraction of total leukocytes (CD45+) in primary DIPG, aGBM, and pediatric cortical tissue samples as calculated via flow cytometry of primary dissociated early autopsy samples. g Lymphocyte fraction of all sampled cells from primary single-cell RNA sequencing of DIPG diagnostic biopsy samples [7]