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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Non-inflammatory tumor microenvironment of diffuse intrinsic pontine glioma

Fig. 1

DIPG and aGBM tumor-associated microglia/macrophages (a-c) Immunofluorescence of primary DIPG tumor tissue (a), primary adult GBM tumor tissue (b), and primary pediatric cerebral cortex (c) for the myeloid cell marker IBA1 (green) and DAPI counterstain (blue). The tumor-associated macrophages in a-b exhibit an amoeboid morphology with shorter processes and enlarged cell bodies, while the normal cortical microglia in c demonstrates the ramified morphology typical of "resting" microglia. Top scale bar = 50μm; bottom scale bar = 10μm (d-e) Representative FACS plots showing CD11b expression against CD45 expression of primary DIPG (d) and aGBM (e) tissue. Samples were gated on size, singularity, and viability prior to these plots (f) Quantification of myeloid fraction (CD11b+) of total leukocytes (CD45+) as calculated by flow cytometry in DIPG, aGBM, and normal cortex primary tissue. ** p < 0.005, *** p < 0.0005 by t-test with Tukey multiple comparison correction

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