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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity

Fig. 6

The effect of (GGGGCC)66 overexpression or C9ORF72 knockdown on TMEM106B protein levels in HeLa cells. a Western blot of HeLa cells transfected with either 2R or 66R pAAV. b-e Protein quantification of TMEM106B (b), LAMP1 (c), CTSD (pro-form, mature form has similar results as the pro-form) (d), and PGRN (e) in cells transfected as described in panel (a). f Western blot of HeLa cells transfected with either control siRNA or siRNA against C9ORF72 (g-j), Protein quantification of TMEM106B (g), LAMP1 (h), CTSD (i), and PGRN (j) in cells transfected as described in panel f. GAPDH was used as a loading control. Graphs represent the mean ± S.E.M. by Student’s t-test (n = 6 for all groups). NS, not significant; *p < 0.05, **p < 0.01

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