Fig. 5

CBTAU-28.1, but not CBTAU-27.1, is capable of immunodepleting seeds from AD brains. Residual seeding activity of human AD brain homogenates following immunodepletion with different concentrations of CBTAU-27.1 and dmCBTAU-27.1 (a) or CBTAU-28.1 and dmCBTAU-28.1 (b) as measured by FRET signal in biosensor cells expressing the microtubule repeat domains of tau (aa 243–375) fused either to yellow or cyan fluorescent protein. Uptake of exogenous tau aggregates into the cells results in aggregation of the tau fusion proteins, which is detected by FRET. As positive and negative controls, a human IgG1 chimeric version of murine anti-PHF antibody AT8 and anti-RSV-G antibody RSV-4.1 were taken along, respectively. For the controls, the same data are shown in plots A and B for visualization purposes. Error bars indicate the SD of two independent experiments