Fig. 4

CBTAU-27.1, but not CBTAU-28.1, inhibits the aggregation of recombinant tau in vitro. Aggregation of rtau in the absence (black) or presence of CBTAU-27.1 (a), dmCBTAU-27.1 (b), Fab CBTAU-27.1 (c), Fab dmCBTAU-27.1 (d), CBTAU-28.1 (e), dmCBTAU-28.1 (f), Fab CBTAU-28.1 (g) or Fab dmCBTAU-28.1 (h), as monitored continuously for 120 h by ThT fluorescence. Three different rtau-IgG (1:0.2 – red, 1:0.4 – blue, and 1: 0.6 – purple) and rtau-Fab (1:0.4 – red, 1: 0.8 – blue, and 1:1.2 – purple) stoichiometries were tested. Each condition was tested in quadruplicate and one representative curve is shown for each condition. For complete datasets, see Additional file 1: Figures S7-S10