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Table 2 Semiquantitative analysis of human aSYN-pathology in distant brain regions

From: A53T-α-synuclein overexpression in murine locus coeruleus induces Parkinson’s disease-like pathology in neurons and glia

   Injected (right) hemisphere
Time of A53T-aSYN overexpression
Non-injected (left) hemisphere
Time of A53T-aSYN overexpression
   1 wk 3 wks 6 wks 9 wks 1 wk 3 wks 6 wks 9 wks
Bregma Main olf. Bulb, gr. layer + ++ ++(+) ++ (+) + + (+)
+4.28 mm Inner plexiform layer +(+) +(+) + (+) (+) (+)
  Outer plexiform layer (+) + + + (+) (+) (+)
Bregma Cortex + ++ ++(+) ++ (+) + + (+)
+ 2.96 mm Main olf. Bulb + ++ ++(+) ++ (+) (+) + (+)
  Ant. olf. Nucleus + +(+) +(+) + (+) (+) (+)
  Lateral olf. Tract (+) +(+) + +
Bregma Cortex + ++(+) ++(+) ++(+) + + +
+ 1.18 mm Taenia tecta, dors. Part + ++ ++ +(+) + + (+)
  Corpus callosum (+) +(+) + + (+) (+) (+)
  Lateral septal nucleus +(+) ++(+) +++ ++(+) (+) + +(+) +(+)
  Diagonal band nucleus +(+) ++(+) +++ ++(+) (+) + +(+) +(+)
  Nucleus accumbens + + + + (+) (+) (+)
  Caudoputamen (+) + + (+) (+) (+) (+)
Bregma Medial septal nucleus + +(+) ++ +(+) (+) + +(+) +
+ 0.38 mm Cortex (+) ++(+) ++(+) ++ + + +
  Bed ncl. of stria term. ++ +++ +++(+) +++ (+) + ++ ++
  Magnocellular ncl. (+) + ++ ++ (+) + +
  Hypothalamus + ++(+) ++(+) +++ +(+) +(+) ++
  Lateral septal nucleus (+) ++ ++(+) ++(+) + +(+) +(+)
  Caudoputamen (+) + + (+) (+) (+) (+)
  Subst. innominata + + +(+) +(+) (+) (+) + +
Bregma Central amygdalar ncl. + ++ +++ +++(+) (+) +(+) ++ ++
−0.58 mm Subst. innominata +(+) ++ ++(+) +++ + +(+) ++ ++
  Globus pallidus (+) + + + (+) (+) (+)
  Hypothalamus + ++ ++(+) ++ (+) + +(+) +(+)
  Lat. hypothalamic area +(+) ++ +++ ++(+) (+) + ++ ++
  Fimbria (+) + (+) (+) (+) + (+) (+)
  Stria terminalis + +(+) +(+) +(+) (+) + +
  Corpus callosum + + + (+) (+)
  Cortex (+) ++(+) ++(+) ++ + + +
Bregma Cortex +(+) +(+) + (+) (+) (+)
−3.16 mm Sup. colliculus, sens. +(+) +(+) + + + +
  Sup. colliculus, motor (+) +(+) ++ ++ (+) +(+) ++ ++
  Periaqueductal gray +(+) ++ ++(+) ++(+) (+) +(+) ++ ++
  Midbrain reticular ncl. +(+) ++ ++ ++(+) + +(+) ++ ++
  Red nucleus (+) + +(+) + + ++ ++(+) ++
  Ventral tegmental area + +(+) ++ ++(+) +(+) ++ ++(+) ++(+)
  Subst. nigra, pars compacta +(+) +(+) ++ ++ (+) + +(+) +(+)
  Subst. nigra, pars reticulata (+) + + (+) (+) (+) (+) (+)
  Hippocampus + +(+) + (+) + +
  Interpeduncular ncl. (+) +(+) ++ ++(+) (+) +(+) ++(+) ++(+)
  Thalamus (+) +(+) ++ ++ (+) + +(+) +(+)
Bregma Dorsal motor ncl. of n. X + +(+) +(+) (+) + +
−7.56 mm Hypoglossal ncl. + + + (+) (+) + (+)
  Parvicell. reticular ncl. +(+) +(+) +(+) +(+) (+) (+) + +
  Intermediate ret. ncl. + + ++ +(+) (+) + +
  Medullary reticular ncl. + +(+) +(+) +(+) + + +
  Lateral ret. ncl. (+) + (+) (+) (+) (+)
  Ncl. of the solitary tract (+) + +(+) (+) + +
  Spinal ncl. of the n. V (+) (+) (+) (+) (+)
  Area postrema (+) (+) + + (+) (+) + +
  Arbor vitae + + + + + +
  Gr. layer (cerebellum) + +(+) +(+) +(+) (+) + + +
  Mol. layer (cerebellum) + + + + + +
  1. Occurrence of human aSYN-positive axons was graded out of seven coronal brain sections as follows: – no positive axons; + sparse (few positive axons); ++ mild (more positive axons); +++ moderate (many positive axons, covering almost the complete brain region) and ++++ severe pathology (large number of positive axons densely covering the complete brain region); (+) describes an intermediate state between two categories to allow a more accurate description. n = 6 per time point. Abundance of aSYN-positive axons increased over time and was more prominent in the injected (right) hemisphere. The signal for human aSYN was solely axonal and no aSYN-positive cell bodies were detected
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