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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Systemic inhibition of the membrane attack complex impedes neuroinflammation in chronic relapsing experimental autoimmune encephalomyelitis

Fig. 4

C6 antisense-mediated MAC inhibition prevents demyelination and microglia/macrophage activation in chronic relapsing EAE. Histological analysis of semi-serial paraffin sections of the mouse cervical spinal cord segment showed lesions in the no drug mice (n = 4) but no signs of active disease in the C6 antisense-treated mice (n = 6). Tissue was collected post-relapse phase. Staining for Luxol fast blue (LFB) and quantification of the signal (a-c) as well as staining for proteolipid protein (PLP) and quantification of the reactivity (d-f) indicated demyelination in the no drug mice (demyelinated lesions pointed by arrows in a and d), but no significant myelin loss in the C6 antisense-treated mice (c, f). Immunostaining for ionized calcium-binding adapter molecule 1 (IBA-1) (g-j) revealed microglia/macrophages within the lesions (arrows in g) and at the peri-lesional area of the no drug mice with a morphology consistent with an activated status. In contrast, all the C6 antisense-treated mice showed amounts of IBA-1+ reactivity similar to the ones found in controls and morphology of IBA-1+ microglia consistent with a resting status (h-j). Moreover, the no drug mice had abundant reactivity for C9, a marker of MAC deposition, within the ventral horns of the spinal cord. In contrast, the C6 antisense-treated mice showed only small amounts of MAC (k-n). Scale bars: (a, b, d, e, g, h, k, m) 200 μm, (i, l) 50 μm. Cresyl violet (CV) was used as counterstain in (a, b). Hematoxylin was used as counterstain in (d, e, g-i, k-m)

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