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Table 1 Bioassay in tga20 mice via intracerebral inoculationa

From: Prion infectivity is encoded exclusively within the structure of proteinase K-resistant fragments of synthetically generated recombinant PrPSc

Experiment

Inoculum

Attack rate (Ndiseased/Ntotal)

Survival time for individual mice (dpi)d

#1

recPrPSc

Cofactors for #1-#3:

• POPG

• Total mouse liver RNA

100% (4/4)

85, 86, 98, 121

#2

recPrPSc

100% (3/3)

91, 93, 94

#3

recPrPSc (after Benzonase+PK digestion)

100% (5/5)

73, 76, 76, 80, 80

#4

recPrPSc

Cofactors for #4-#6:

• POPG

• Synthetic Poly rA RNAc

100% (4/4)

104, 109, 115, 119

#5 b

recPrPSc

100% (4/4)

95, 95, 104, 110

#6 b

recPrPSc (after Benzonase+PK digestion)

100% (4/4)

95, 106, 108, 109

  1. aDifferent batches of recPrPSc were used
  2. bThe same batch of recPrPSc was used to for these two experiments
  3. cSee reference [37]
  4. ddpi: days post injection