Fig. 3

PK digestion did not reduce prion infectivity. a RecPrP (left panel) and the sPMCA product (right panel) were subjected to ultracentrifugation (100,000 × g, 1 h, 4 °C) with or without prior PK digestion (50 μg/mL, 30 min, 37 °C) as indicated (T: total input, 1 μL; S: supernatant fraction, 1 μL; P: pellet fraction, 1 μL). For PK digestion, 10 μL of sample from the supernatant or pellet fraction was used. b The same batch of recPrP^Sc was untreated or was treated with Benzonase, PK, or both, as indicated. c Prion infectivity of samples in (b) was evaluated by Elispot cell infection assay