Fig. 2
Prion infectivity and the amount of PK-resistant recPrP conformer increased concurrently. a Upper panel: an illustration of recPrPSc amplification in sPMCA. Lower panel: detection of PK-resistant recPrP at 0, 2, 4, 6, 8, 10, 12, and 24 h within a single round. b The infectivity of sPMCA products collected in (a) was evaluated by Elispot CAD5 cell infection assay. c and d The amplification of the PK-resistant recPrP conformation correlated with prion infectivity. The percentage increase of cells positive for PK-resistant PrPSc (red dots, left Y axis) and of PK-resistant recPrPSc (green triangles, right Y axis) within a single round of sPMCA were plotted as a function of time (h) (c). The percentage increase of cells positive for PK-resistant PrPSc was plotted versus the percentage increase of PK-resistant recPrPSc (d). The linear regression analysis was performed and a linear regression line (dashed line) was added to the plot (Equation: Y = 1.156*X + 10.19, R2: 0.92)