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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Genetic deletion of muscle RANK or selective inhibition of RANKL is not as effective as full-length OPG-fc in mitigating muscular dystrophy

Fig. 3

Full-length OPG-Fc, but not muscle RANK deletion, prevents eccentric contraction-induced muscle dysfunction. a Ex vivo eccentric contraction protocol of EDL muscles were performed on 5-week-old C57BL/6, mdx-RANKmko and mdx mice treated from days 25 to 35 with vehicle (PBS) or full-length OPG-Fc [1 mg/kg/d]. Full-length OPG-Fc, but not muscle RANK deletion, significantly prevented the loss of force following repetitive eccentric contractions. Functional performance was assessed on 5–6-month-old mdx mice treated with vehicle (PBS) or full-length OPG-Fc [1 mg/kg/d] for 10 days prior downhill running. b Only 10% of the PBS-treated versus 75% of the full-length OPG-Fc treated mdx mice were able to complete the downhill running protocol at a speed of 10 m/s for 45 min. c The total distance travelled at 10 m/min for 45 min (450 m) for each experimental group. d The full-length OPG-Fc treatment rescued the voluntary cage activity post- exhausting eccentric downhill running. Data are shown as mean values +/− s.e.m.; one way ANOVA and Tukey’s post-hoc; significantly different at (a) each contraction or (b-d) each time point from PBS-treated mdx mouse; * p < 0.05, ** p < 0.01, *** p < 0.001

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