Table 1 Inactivation effects of pathogenic α-syn derived from recombinant α-syn protein and MSA patients’ brains in SH-SY5Y cells
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Treatment | Synthetic α-syn fibrils | MSA-2 Pu | MSA-3 FC |
|---|---|---|---|
No treatment | 100 (± 11.22) | 100 (± 3.38) | 100 (± 11.49) |
1% SDS | 65.5 (± 16.48) | 48.6 (± 18.66) | 13.8 (± 2.96) |
100 °C 3 min | 109.7 (± 5.00) | 114.2 (± 20.45) | 29.5 (± 2.07) |
120 °C 20 min | 45.3 (± 14.75) | 75.1 (± 13.45) | 17.5 (± 2.37) |
120 °C 20 min/0.1% SDS | 44.9 (± 4.45) | not tested | not tested |
120 °C 20 min/1% SDS | 3.3 (± 2.94) | 10.5 (± 2.91) | 1.7 (± 1.23) |
134 °C 20 min | 8.7 (± 5.31) | 19.0 (± 2.13) | 6.3 (± 0.93) |
134 °C 20 min/0.1% SDS | 11.5 (± 2.31) | not tested | not tested |
134 °C 20 min/1% SDS | 2.8 (± 2.76) | 3.5 (± 0.95) | 6.8 (± 0.25) |