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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods

Fig. 7

Inactivation of synthetic α-syn fibrils by SDS treatment and autoclaving. a Synthetic α-syn fibrils were subjected to various inactivation treatments and analyzed by immunoblotting with Syn 102–116 and anti-syn 131–140 antibodies (upper). Treated α-syn samples were treated with protease K (5 μg/mL) and analyzed by immunoblotting (lower). b α-Syn fibrils were subjected to various inactivation treatments (2 μl) and introduced into SH-SY5Y cells transiently expressing human WT α-syn. Immunoblot analysis of sarkosyl-insoluble fractions (ppt) and sarkosyl-soluble fractions (sup) extracted from mock-transfected cells and cells transfected with α-syn monomer and fibrils, and treated with 1% SDS for 1 h at room temperature, or boiled, or autoclaved (AC) at 120 °C with or without 0.1%, 1% SDS, or autoclaved at 134 °C with or without 0.1% or 1% SDS are shown. Phosphorylated α-syn was detected with anti-phosphorylated α-syn PSer129 antibody. α-Syn was detected with anti-syn 131–140 antibody. c Quantification of immunoblot analysis shown in b. The results are expressed as means ± SEM (n = 3). “No treatment” was taken as 100%. One-way ANOVA with Dunnett’s post hoc test were used for multiple comparisons to no treatment, *P < 0.05; **P < 0.01

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