Fig. 5
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Dose-dependent propagation following introduction of different amounts of synthetic α-syn fibrils in non-Tg mice. a Various amounts of mouse α-syn fibrils inoculated into WT mice dose-dependently induced α-syn pathology in frontal cortex, striatum, amygdala and substantia nigra. α-Syn pathology in mouse brains inoculated with 40, 10, 1, and 0.1 μg of α-syn fibrils into striatum at 3 months after inoculation is shown. The numbers of injected mice are shown in Additional file 5: Table S2A. Sections were evaluated by immunohistochemistry with PSer129 antibody EP1536Y. Scale bar, 100 μm. b Quantification of α-syn pathology in brains of mice inoculated with different amounts (40 μg to 10 pg) of mouse α-syn fibrils into striatum. The box plots show the number of pS129-positive cells in different regions. One-way ANOVA with Dunnett’s post hoc test was used for multiple comparisons to 10 pg, *P < 0.05; **P < 0.01. FC: frontal cortex, Str: striatum, Amy: amygdala, SN: substantia nigra