Fig. 4
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Seed-dependent α-syn aggregation induced in SH-SY5Y cells by serial dilutions of insoluble fractions extracted from brains of patients with synucleinopathies. Serial dilutions of sarkosyl-insoluble fractions prepared from cerebellum, frontal cortex and putamen of 3 MSA cases and frontal cortex of a DLB case (2 μl) were introduced into SH-SY5Y cells transiently expressing human WT α-syn. Quantification of phosphorylated α-syn accumulated in SH-SY5Y cells induced by serial dilutions of pathogenic α-syn derived from brain samples. Band intensities of immunoblot analysis shown in Additional file 4: Figure S3 were measured. The results are expressed as means ± SEM (n = 3). Cb: cerebellum, FC: frontal cortex, Pu: putamen, TC: temporal cortex