Fig. 3
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Prion-like properties in SH-SY5Y cells of α-syn aggregates extracted from brains of synucleinopathy patients. a Sarkosyl-insoluble fractions extracted from patients’ brains (2 μl) were introduced into SH-SY5Y cells transiently expressing human WT α-syn. Immunoblot analysis of sarkosyl-insoluble fractions (ppt) and sarkosyl-soluble fractions (sup) extracted from mock-transfected cells, and sarkosyl-insoluble fractions from cerebellum, frontal cortex and putamen of 3 MSA cases, frontal cortex and temporal cortex of 4 DLB cases, control brain and an AD case. Phosphorylated α-syn was detected with anti-phosphorylated α-syn PSer129 antibody. α-Syn was detected with anti-syn 131–140 antibody. The α-syn concentrations of Sarkosyl-insoluble fractions derived from human brains are shown in Additional file 1: Table S1A. b Quantification of immunoblot analyses shown in A. The results are expressed as means ± SEM (n = 3). *P < 0.05. c SH-SY5Y cells into which synthetic human α-syn fibrils (2 μg) or sarkosyl-insoluble fractions from cerebellum of a MSA case and temporal cortex of a DLB case (2 μl) had been introduced were fixed and immunostained with PSer129 antibody EP1536Y. Scale bar, 100 μm. Cb: cerebellum, FC: frontal cortex, Pu: putamen, TC: temporal cortex