Fig. 10
From: Potent prion-like behaviors of pathogenic α-synuclein and evaluation of inactivation methods
Effects of inactivation treatments of synthetic α-syn fibrils on seeding activity in non-Tg mice. a 2 mg/ml mouse α-syn fibrils were exposed to various treatments (1% SDS for 1 h at room temperature, boiling, autoclaving (AC) at 120 °C with or without 0.1% SDS or autoclaving at 134 °C with or without 0.1% SDS), and 5 μl aliquots of the resulting samples were inoculated into WT mice. α-Syn pathologies in frontal cortex, striatum, amygdala and substantia nigra at 3 months after inoculation are shown. The numbers of injected mice are shown in Additional file 5: Table S2B. Sections were evaluated by immunohistochemistry with PSer129 antibody EP1536Y. Scale bar, 100 μm. b Quantification of α-syn pathology in mouse brains inoculated into striatum with α-syn fibrils exposed to various treatments. The box plots show the numbers of pS129-positive cells in different regions. One-way ANOVA with Dunnett’s post hoc test were used for multiple comparisons to no treatment, *P < 0.05; **P < 0.01. FC: frontal cortex, Str: striatum, Amy: amygdala, SN: substantia nigra