Fig. 5
From: Prion-like propagation of β-amyloid aggregates in the absence of APP overexpression
Characterization of induced Aβ deposits in AppNL-F mice inoculated with purified TgCRND8 Aβ aggregates. a Brain sections from male AppNL-F mice at 130–132 dpi with either BSA or purified TgCRND8 Aβ aggregates were analyzed by immunohistochemistry for Aβ40 (11A50-B10 antibody), Aβ42 (12F4 antibody), or Aβ43 (9C4 antibody), or by staining with Thio S. Brain sections from 20-month-old uninoculated male AppNL-F mice were used as a control. Representative images of the corpus callosum from inoculated mice and the cortex of uninoculated mice are shown. Cortical Aβ deposits were used for comparison since the Aβ pathology within the corpus callosum of aged, uninoculated AppNL-F mice is relatively mild compared to the robust Aβ deposition present in the cortex. Scale bar: 20 μm (applies to all images). b Quantification of Aβ40, Aβ42, and Aβ43 staining (percentage area stained; mean ± s.d.) in the corpus callosum of AppNL-F mice at 130 dpi with purified TgCRND8 Aβ aggregates (n = 4). ***P < 0.001