Fig. 4
From: Prion-like propagation of β-amyloid aggregates in the absence of APP overexpression
Induction of cerebral Aβ deposition in AppNL-F mice by inoculation with purified TgCRND8 Aβ aggregates. a Aβ immunohistochemistry with the 4G8 antibody on brain sections from male AppNL-F mice at 132 dpi with BSA, or at 14 or 130 dpi with purified TgCRND8 Aβ aggregates. Representative images of the corpus callosum and olfactory bulb from two independent mice per inoculation group are shown. Scale bars: 20 μm. b Quantification of Aβ staining (percentage area stained; mean ± s.d.) in the corpus callosum and olfactory bulb of AppNL-F mice inoculated with either BSA (n = 5) or purified TgCRND8 Aβ aggregates (n = 4) at 130–132 dpi. ***P < 0.001, **P < 0.01. c Total (formic acid-extractable), PK-resistant, and soluble (diethylamine-extractable) Aβ42 levels (mean ± s.d.) in brain homogenates from male AppNL-F mice at 130–132 dpi with either BSA (n = 5) or purified TgCRND8 Aβ aggregates (n = 4) as well as total Aβ42 levels from mice at 14 dpi with purified TgCRND8 Aβ aggregates (n = 4) were determined by ELISA. **P < 0.01; *P < 0.05; ns, not significant