Fig. 3
From: Prion-like propagation of β-amyloid aggregates in the absence of APP overexpression
Purification of Aβ aggregates from the brain of an aged TgCRND8 mouse. a The purity of the isolated TgCRND8 Aβ aggregates was analysed by SDS-PAGE followed by either silver staining (left) or immunoblotting with the anti-Aβ antibody 6E10. b The Aβ isoform composition of the purified TgCRND8 Aβ aggregates was determined by separation on a Bicine/Tris 8 M urea gel followed by silver staining. PK-resistant Aβ fibrils prepared from various recombinant Aβ isoforms were used as controls. c Ultrastructural analysis of purified TgCRND8 Aβ aggregates by electron microscopy. Scale bars: 500 nm (left image) or 100 nm (right image)