Fig. 1From: Integrated proteomics and network analysis identifies protein hubs and network alterations in Alzheimer’s diseaseAD-associated brain proteome changes revealed by label-free quantitative proteomics. a Volcano plot displaying the distribution of all proteins (n = 1968) with relative protein abundance (log2 AD/control ratio) plotted against its significance level (negative log10 P-value), showing significantly (P < 0.05) increased (AD/control ratio > 1.3; Green) and decreased (AD/control ratio < 0.77; Red) proteins in AD. b Heat map representation of 16 individual sample abundances for 487 significantly altered proteins after unsupervised hierarchical clustering, segregating samples into AD (left) and controls (CT; right) and proteins into up-regulated (top) and down-regulated (bottom) proteins in AD. c-g Western blot analysis (c, e) and quantification (d, f, g) confirm the decreased expression of STK39 (c, d) and increased expression of Smac proteins (e-f) in AD versus control. Data represent mean ± SEM (error bars; n = 8 biological repeats for AD or control group). *, P < 0.05; **, P < 0.01, unpaired two-tailed Student’s t test. Each experiment was repeated three times with similar resultsBack to article page