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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Rapid detection of 2-hydroxyglutarate in frozen sections of IDH mutant tumors by MALDI-TOF mass spectrometry

Fig. 2

2HG quantification of validation set. a MALDI-TOF spectra showing m/z values from 146.75 to 148.75 of IDH wildtype and IDH mutant tissue with the matrices 1,5-DAN. Peaks at m/z 147 represent 2HG. The colors of the spectra correlate with the colours shown in Additional file 1: Table S1. b Box plots of MALDI-TOF signal intensities of IDH wildtype and IDH mutant tissue at m/z 147. Statistical analysis was performed with Wilcoxon-Mann-Whitney-Test (p < 0.0001). Error bars represent the standard deviation. c D-2HG concentrations of the validation set measured by the biochemical assay. d Box plots of D-2HG concentrations of IDH wildtype and IDH mutant tissue. Statistical analysis was performed with Wilcoxon-Mann-Whitney-Test (p < 0.0001). Error bars represent the standard deviation. e Correlation plot of MALDI-TOF intensities and biochemical determined D-2HG concentrations. Correlation was determined by Pearson (r = 0.862, p < 0.0001). f Normalized MALDI-TOF values of validation set. Values shown are intensities divided by the total ion count of each corresponding spectra

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