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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: CD74 regulates complexity of tumor cell HLA class II peptidome in brain metastasis and is a positive prognostic marker for patient survival

Fig. 6

Effects of siRNA mediated CD74 knockdown on the HLA class II peptidome of H1 brain metastatic melanoma cells. a Summed MS1 intensities and (b) Number of identifications of HLA class II ligands in label-free quantitation mass spectrometry (error bars represent standard error of the mean (SEM). c Volcano plot of differentially presented source proteins in CD74 knockdown vs control (d) DAVID Functional Annotation Clustering of differentially represented HLA class II source proteins. e Schematic illustration of CD74 functions (left) and consequences of CD74 knockdown/downregulation (right) in brain metastatic tumor cells. 1: HLA class II and invariant chain complex in the endoplasmic reticulum and Golgi apparatus, 2: HLA class II compartment, 3: Processing of invariant chain by proteases, CLIP fragment remains and is exchanged for an antigenic peptide, 4: Complex antigens are expressed on the tumor cell surface when CD74 is highly expressed, 5: Tumor cell - CD4-positive lymphocyte interaction, 6: Recruitment of CD8-positive T-cells, 7: direct lysis by CD8 or 8: CD4-positive cells. Dotted lines illustrate impaired tumor cell – lymphocyte interactions. XXX denote HLA class II ligands. Multiple colors of ligands denote high peptidome complexity

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