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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: CD74 regulates complexity of tumor cell HLA class II peptidome in brain metastasis and is a positive prognostic marker for patient survival

Fig. 5

The influence of CD74 siRNA mediated knockdown on HLA class I and II machinery in H1 brain metastatic melanoma cells. a Immunocytochemistry of H1 melanoma cells against CD74, HLA class II molecules and Ki67. b + c Western blot analysis of siRNA-mediated CD74 knock-down versus siRNA-control with (c) protein quantification normalized against Lamin B1. d qRT-PCR analysis of siRNA-mediated CD74 knock-down versus control. e FACS analyses against HLA class II molecules on H1 melanoma cells. Differential transcript expression of H1 siRNA CD74 versus control siRNA (f, g). f TaqMan® array focusing on the transcriptome of the human antigen processing and presentation machinery by HLAs, (g) excerpt of micro-array data with regard to HLA class I and II transcripts

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