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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Preventing mutant huntingtin proteolysis and intermittent fasting promote autophagy in models of Huntington disease

Fig. 5

Scheduled feeding alters nutrient-sensing pathways in the brain. a - c YAC128 mice and their wt littermates were subjected to 1 week of scheduled feeding and compared to littermates with ad libitum access to food. a Levels of total and phosphorylated mTOR were analyzed by Western blot. 2way-ANOVA genotype p = 0.6796, feeding p = 0.0082. b + c mRNA levels of SIRT1 (b) and p62 (c) were analyzed by qRT-PCR. SIRT1: 2way-ANOVA genotype p = 0.7651, feeding p < 0.0001, p62: 2way-ANOVA genotype p = 0.4121, feeding p < 0.0001. Representative blots and pooled quantification data with S.E.M. are shown. Statistical significance was determined by 2way-ANOVA with Bonferroni’s post-hoc correction, number of replicates is shown as insets. *: p < 0.05, **: p < 0.01, ***: p < 0.001

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