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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Preventing mutant huntingtin proteolysis and intermittent fasting promote autophagy in models of Huntington disease

Fig. 4

mHTT levels increase in aging YAC128 liver and can be reduced by fasting-induced autophagy. a, b + d 12 month old YAC128 and C6R mice, as well as their wt littermates, were subjected to a 24 h fasting period, sacrificed immediately and liver samples were compared to littermates with ad libitum access to food. p62 (a) and LC3 (b) protein levels were analyzed by Western blot. p62: 2way-ANOVA genotype p = 0.0103, fasting p < 0.0001, LC3-I: 2way-ANOVA genotype p = 0.0043, fasting p = 0.3161, LC3-II: 2way-ANOVA genotype p = 0.2012, fasting p = 0.0151. c Liver tissues from YAC128 and C6R mice at different ages were analyzed for HTT expression using the MAB2166 antibody. mHTT 2way-ANOVA genotype p = 0.0047, age p = 0.0342; wt HTT 2way-ANOVA genotype p = 0.3168, age p = 0.0232. d HTT protein levels in YAC128 liver were analysed by Western blot using the MAB2166 antibody. Representative blots and pooled quantification data with S.E.M. are shown, number of replicates is shown as insets. Statistical significance was determined by 2way-ANOVA with Bonferroni’s post-hoc correction for a - c, or two-tailed Student’s t-test for d. *: p < 0.05, **: p < 0.01, ***: p < 0.001

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