Fig. 1
MRI reliably detects de- and re-myelination events in the cuprizone model in the corpus callosum/external capsule, correlating with myelin and oligodendrocyte histology. a Representative MRI images acquired from two mice, one receiving normal food (left) and the other treated with 0.2% cuprizone for 5 weeks with subsequent switch to normal food. b Representative pictures from histological and immunohistological stainings from the corpus callosum/external capsule after 5-week 0.2% cuprizone food and control food treatment and recovery for 2 and 4 weeks (switch to normal food) of C57BL/6 mice detecting myelin by Luxol fast blue (LFB), myelin oligondendrocyte glycoprotein (MOG) and GST-π positive oligodendrocyte cells in the corpus callosum/external capsule. c Correlation analysis of quantitative histology (LFB and MOG optical density (OD), GST-π positive soma area normalized (norm.) to region of interest) and MRI signal/MTR parameters. Two-tailed Pearson correlation analysis, correlation coefficients (R2) and p values are indicated. Scale bars: 400 μm. cc: corpus callosum, ec: external capsule, MRI: magnetic resonance imaging, MTR: magnetization transfer ratio, a.u.: arbitrary units, OD: optical density