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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Lack of pathogenic potential of peripheral α-synuclein aggregates from Parkinson’s disease patients

Fig. 2

Integrity of the nigrostriatal pathway in LB-SG-injected mice. a Schematic diagram indicating the site of nigral stereotactic inoculations to mice. b Contralateral forepaw use, as assessed by the cylinder test, in mice injected with control- and PD-derived LB-SG fractions at 6 months post-inoculation. Ctrl: n = 8; mean = 47.87; SD = 9.128; SEM = 3.227; LB-SG: n = 12; mean = 52.62; SD = 7.929; SEM = 2.289; p = 0.23, two-tailed t-test. c Left, representative photomicrographs of ipsilateral tyrosine hydroxylase (TH)-positive substantia nigra pars compacta (SNpc, outlined in red) neurons (dark brown; thionin staining in purple) from vehicle-, Ctrl- and LB-SG-injected animals, at 6 months post-inoculation. Right, quantification of ipsilateral TH-positive SNpc neurons by stereology in vehicle-, Ctrl- and LB-SG-injected mice, at 6 months post-inoculations. Vehicle: n = 9; mean = 5736.66; SD = 879.61; SEM = 293.20; Ctrl: n = 7; mean = 5198.57; SD = 1282.38; SEM = 484.69; LB-SG: n = 9; mean = 5520; SD = 1219.34; SEM = 406; p = 0.644, one-way ANOVA. d Left, representative photomicrographs of TH-positive striatal terminals (in brown) from vehicle-, Ctrl- and LB-SG-injected animals at 6 months post-inoculation (asterisk indicate injected hemisphere). Right, quantification of ipsilateral TH-positive striatal terminals by optical densitometry (OD) in Ctrl- and LB-SG-injected mice, relative to vehicle-injected animals, at 6 months post-inoculations. Ctrl: n = 8; mean = 96.86; SD = 9.52; SEM = 3.37; LB-SG: n = 11; mean = 97.39; SD = 18; SEM = 5.427; p = 0.836, Mann-Whitney rank sum test. e Quantification of microglial Iba1 and astrocytic GFAP reactions by OD in the ipsilateral SN of Ctrl- and LB-SG-injected mice, relative to vehicle-injected animals, at 6 months post-inoculations. For Iba1, Ctrl: n = 8; mean = 119.8; SD = 16.04, SEM = 5.671; LB-SG: n = 11; mean = 120.7; SD = 12.56; SEM = 3.788; p = 0.773 Mann-Whitney rank sum test. For GFAP, Ctrl: n = 8; mean = 99.77; SD = 14.3; SEM = 5.055; LB-SG: n = 12, mean = 113.7; SD = 17.67; SEM = 5.101; p = 0.08, two-tailed t-test. In all panels, histograms represent average ± standard error of the mean (SEM). Vehicle-injected mice, n = 9–11. Scale bars, 500 μm (c) and 1 mm (d)

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