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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Lack of pathogenic potential of peripheral α-synuclein aggregates from Parkinson’s disease patients

Fig. 1

Purification of peripheral Lewy bodies (LB) from stellate ganglion (SG) of PD patients. a Schematic representation of SG localization and representative photomicrograph of α-synuclein pathology by immunohistochemistry (in brown) in the SG of one of the PD patients used in this study, as previously reported [8]. Arrows, LB-like structures; asterisks, Lewy neurites. b Left, schematic representation of the sucrose gradient fractionation procedure used to purify LB-containing fractions from freshly frozen human SG tissue. Right, filter retardation assay probed with a human α-synuclein antibody to assess the presence of insoluble α-synuclein aggregates in the different fractions obtained by sucrose gradient fractionation from the SG of three sporadic PD patients and two non-PD control (Ctrl) subjects. Rectangles indicate the fractions selected to prepare the mixture used for inoculations. c Immunoblot levels of human α-synuclein in fractions 16 to 18 (corresponding to the 1.4/2.2 M interface) from PD patients and non-PD control subjects

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