Fig. 1

Detection of αSyn seeding activity in BH and CSF using K23Q (blue), WT (red) and WT* (green; commercial wild-type rαSyn lacking a 6× histidine tag [7]) substrates. Reactions were seeded in quadruplicate with brain homogenate (BH) (top panel) or CSF (bottom panel) from Parkinson’s disease (PD) or non-synucleinopathy (NS) including corticobasal degeneration cases (CBD BH) and healthy donors (NS CSF). For the BH assays, 10^− 3 (closed symbols) or 10^− 4 (open symbols) brain tissue dilutions from a single PD or NS case were used. For the CSF assays, 15 μl (undiluted) from two PD cases and one NS case was used. Each sample trace represents the average ThT signal of quadruplicate wells. For clarity only every other data point is plotted. The vertical dashed line designates the assay cutoff time used in subsequent analyses