Fig. 7
Neurotrophic support in PLP-α-syn mice. a GDNF protein levels of 2 months old MSA mice as compared to the controls by two way ANOVA indicated no general effect of region and genotype: effect of genotype F1,50 = 1.09, p = 0.30224, effect of region F4,50 = 0.83, p = 0.5118, interaction F4,50 = 0.93, p = 0.4564), (b) At 12 months of age, GDNF levels get higher in the striatum of the transgenic mice than in the controls, possibly reflecting compensatory glial activation at this stage (two way ANOVA indicates a general effect of the genotype: effect of genotype F1,49 = 9.58, p = 0.0.0033, effect of region F4,49 = 1.27, p = 0.2964, interaction F4,49 = 1.25, p = 0.3032). c BDNF shows decreased protein levels in the striatum and brainstem of MSA mice compared to age-matched counterparts at 2 months of age (two way ANOVA indicates a general effect of region and genotype: effect of genotype F1,50 = 6.88, p = 0.0115, effect of region F4,50 = 4.2, p = 0.0052, interaction F4,50 = 4.2, p = 0.0052); (d) At 12 months of age, the difference in the brainstem remains, while the one in the striatum loses its significance, possibly linked to compensatory mechanisms related to glial activation (two way ANOVA indicates a general effect of genotype: effect of genotype F1,49 = 7.42, p = 0.0089, effect of region F4,49 = 0.58, p = 0.6771, interaction F4,49 = 0.58, p = 0.6771); ** p < 0.01, ***p < 0.001 compared to age-matched healthy controls. Band size: BDNF, 14 kDa; GDNF, 15 kDa; actin, 40 kDa