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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Iatrogenic Creutzfeldt-Jakob disease with Amyloid-β pathology: an international study

Fig. 2

WB profile of resPrPSc from iCJD and sCJD controls using high resolution gel electrophoresis. BHs from the frontal cortex (lanes 8 and 9) and cerebellum (lanes 1–7) treated with 100 U/ml PK (~2000 μg/ml) were probed with 3F4. PrP bands were resolved in a 15% Tris–HCl, 20 cm-long gel, and visualized with the near-infrared LI-COR system. The resPrPSc profiles from iCJD (lanes 1, 3, 5, 7 and 8) and matching sCJD subtypes (lanes 2, 4 and 6) are similar as shown by the co-migration of the unglycosylated resPrPSc type 2 (19) (lanes 1–4) and type 1 (21) (lanes 6–8). The different thickness of the type 2 (19) band is within the variability range for this subtype. The unglycosylated resPrPSc band of ~20 kDa, also identified as type i (i), is visible in GH-iCJDMVi + 2 (lane 3), sCJDMVi + 2 (lane 4) and GH-iCJDMMi case (lane 5); a thin band of ~20 kDa (arrowhead) is also present in CJDMM1. An ~18 kDa fragment (18) is also present in CJDVV2 and CJDMVi + 2

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