Fig. 5

Rescue of Munc18–1-knockdown-induced migration defects by Syntaxin1A. a pCAG-RFP was coelectroporated in utero with pSuper-H1.shLuc (Control) + pCAG-Myc vector (i), or with sh-Munc#1 together with pCAG-Myc (ii), −Myc-Syntaxin1A (iii), −Myc-Syntaxin1B (i’) or -Myc-Syntaxin1AB chimera (ii’) into VZ cells at E14.5, followed by fixation at P2. Coronal sections were stained for RFP (red) and nuclei (blue). Dotted lines represent the pial surface. Bar, 100 μm. b Quantification of the distribution of RFP-positive neurons in distinct regions of the cerebral cortex for each condition in (a). Error bars indicate SD (i, n = 5; ii, n = 7; iii, n = 7; i’, n = 5; ii’, n = 10); *p < 0.05, **p < 0.01 by Tukey-Kramer LSD. c Detection of Myc-Syntaxin1A (i), −Syntaxin1B (ii) and -Syntaxin1AB chimera (iii). Electroporation was done as in (a). After fixation, cells were immunostained for RFP (red) and Myc (green). Bar, 10 μm