Fig. 6
From: α-Synuclein fibrils recruit peripheral immune cells in the rat brain prior to neurodegeneration
α-Synuclein fibril exposure in the SNpc induces progressive inflammation and inclusion formation in the dorsal striatum. a Flow cytometry analysis of live CD45hi, CD11b-expressing monocytes and macrophages in dorsal striatum tissue isolates from 45 rats bi-laterally injected with either saline, monomer, or α-synuclein fibrils and analyzed eight-weeks post injection. b Quantification of microglia, eight-weeks post-injection, and those that are (c) expressing MHCII in the dorsal striatum. Scatter plots are presented in Additional file 1: Figure S3. d Unbiased stereological estimates of p-Ser129-α-synuclein inclusions in the ipsilateral dorsal striatum one, three, and six months post-injection monomer (blue bars) or fibrils (yellow bars) injections. Data from 60 rats is shown. e-g From the rats analyzed in panel d, representative immunofluorescence depicting inflammation in the ipsilateral dorsal striatum caused by monomer injections or (h-j) fibril injections. Confocal images are shown with MHCII or CD163 as green color, IBA-1 as red color, and pSer129-α-synuclein as blue color. Scale bars are 40 μm, with white boxes showing zoom insets. Column graphs show group mean values and error bars show SEM. Data points represent mean values from individual rats. *p<0.05, Tukey’s post-hoc test and one-way ANOVA. n.s. is not significant