Fig. 4
From: α-Synuclein fibrils recruit peripheral immune cells in the rat brain prior to neurodegeneration
α-Synuclein fibrils promote microglial activation (but not proliferation) and peripheral immune cell infiltration in the SNpc. a Flow cytometry analysis of CD45hi, CD11b-expressing monocytes and macrophages in ventral midbrain isolates from 45 rats injected with either saline (A1), monomer (A2), or α-synuclein fibrils (A3), bi-laterally, eight-weeks post injection. (A4) Analysis of monocytes and macrophages based on CD45hi expression in live cells (see Additional file 1: Figure S2 for full gating strategy). b Flow cytometry analysis of CD45hi, CD11b, MHCII positive cells (inflammatory monocytes and macrophages) in ventral midbrain isolates from saline (B1), monomer (B2), and α-synuclein fibril (B3) injected rats. (B4) Quantification of high MHCII-expressing cells in the monocyte and macrophage pool. c Quantification of monocytes/macrophages in ventral midbrain isolates, determined by CD45Hi and CD11b expression. d Quantification of the number of CD45int, CD11b expressing microglia in ventral midbrain isolates. e Quantification of CD4-expressing T cells. Graphs show columns with mean values and error bars representing SEM. N=15 rats analyzed per group. *p<0.05, Tukey’s post hoc test. n.s. is not significant (one-way ANOVA). Scatterplots shown are representative of the groups