Fig. 3

α-synuclein fibrils, but not monomer exposures, result in a sustained MHCII and CD163+ cell response in the SNpc. Representative confocal images of the rat SNpc (coronal sections). MHCII (green color), IBA-1 (red color) and pSer129-α-synuclein (blue color) signal was evaluated in monomer-injected rats 1 month (a), 3 months (b), and 6 months (c) post injection, and in fibril-injected rats 1 month (d), 3 months (e), and 6 months (f) post injection. CD163 stain (green color) with IBA-1 (red color) was evaluated in serial sections from the same rats (panels g-l). CD163+ cells shown are outside of the vasculature and therefore not perivascular macrophages. Scale bar is 30 μm, and white boxes are zoom insets with a scale bar of 10 μm. Images are representative of the SNpc in at least three rats analyzed per group