Fig. 1
From: α-Synuclein fibrils recruit peripheral immune cells in the rat brain prior to neurodegeneration
α-Synuclein fibril exposure promotes antigen processing, presentation and activation in primary microglia. a Dynamic light scattering analysis and b circular dichroism of monomeric (blue) and fibril (yellow) preparations of α-synuclein. c Representative electron microscopy photomicrograph of α-synuclein fibrils. Scale bar is 100 nm. d Representative confocal images of MHCII and e iNOS immunohistochemistry of primary microglia cells treated with 1 μg mL-1 α-synuclein monomer or fibrils for four hours. f DQ-Ovalbumin fluorescent images of primary microglia treated with α-synuclein monomer and fibrils for thirty minutes prior to imaging. Scale bar is 50 μm. g Quantification of MHCII, iNOS, and DQ-Ovalbumin fluorescence in primary microglia. Mean values are calculated from at least four different photomicrographs from three independent experiments. **p<0.05, one-way ANOVA and Tukey’s post hoc test