Fig. 4

pS1292-LRRK2 levels in urine and saturation in CSF Absolute levels of pS1292-LRRK2 were normalized to total LRRK2 protein to derive a ratio of the percent of total LRRK2 that is autophosphorylated. a Scatter plots showing percent pS1292-LRRK2 from urinary exosomes (N=132, blue: male, red: female). b Scatter plots showing percent pS1292-LRRK2 from CSF exosomes (N=81, blue: male, red: female). Values above 100% phosphorylation were considered within technical error of detection of full saturation and plotted as 100% for visualization purposes only and not for statistical analysis. Unadjusted absolute values of pS1292-LRRK2 are given in Fig. 5. Bars show median values. ***p-value<0.001, **p-value<0.01, *p-value<0.05, ns: p-value>0.05. p value between groups were calculated using Dunn’s multiple comparison test (a and b)