Fig. 4
From: The chronically inflamed central nervous system provides niches for long-lived plasma cells
Long-lived plasma cells with non-neuronal or non-self specificities for neuroinflammation persist in the chronically inflamed CNS only to a very low extent. a The scheme demonstrates the experimental procedure for EdU pulse-chase experiment starting after boost with additional application of ovalbumin (OVA). The mice were immunized and boosted with rhMOG and OVA. Afterwards, they received EdU for 14 days via drinking water. Analysis was performed directly after stopping the EdU-feeding or five weeks after boost. b A representative confocal image of spinal cord from day 42 after boost is shown. Signals after immunofluorescence staining of antibody-secreting cells (κ, green), DAPI (left, blue), EdU (red) and OVA (right, blue) are shown. Data of four mice pooled from two independent experiments are shown. Scale bar scan represents 50 μm