Fig. 2

Characterization of EAE-boost model. Mice were immunized and boosted (day 28) with rhMOG. a Daily clinical EAE scores are shown. Graph depicts mean value and standard deviation of 48 animals pooled from ten independent experiments. b The clinical outcome during the first (control group) and the second (boost group) peak was directly compared. Graph depicts mean value and standard deviation of 48 animals pooled from ten independent experiments. c The day of onset was compared between the first (control group) and the second (boost group) peak. The differences between the groups were tested with the Log-Rank (Chi Square) test (***P < 0,001). d Graphs demonstrate relative standard concentration of total (left) and MOG-specific immunoglobulin (right) in serum of mice untreated, immunized or boosted, analyzed in the chronic phase. Bars represent mean, each dot represents one mouse pooled from eight (immunized) and five (boost) individual experiments. The statistical differences were tested with the unpaired Mann-Whitney U test (*P < 0,05, ***P < 0,001)