Fig. 6

Altered localization of C760R mutant protein. Immunohistochemical analyses with anti-DINE antibody in horizontal sections of E12.5 mouse spinal cords (a–i) and diaphragm muscles (j–r). In the case of wild-type spinal cord, DINE immunoreactivity was detected in both motor neuron soma and axons (arrows), which were labeled with GFP (a–c). Similar immunoreactivity could be detected at the end of the phrenic motor nerves innervating diaphragm muscle (j–l). In contrast, DINE expression was drastically decreased in C760R (d–f, m–o) as well as C760G motor axons (g–i, p–r)