Fig. 5

RaPrnp vector drives expression in rat neurons. Visualization of the LacZ/EGFP dual-reporter at the cellular level in a 1-year-old Tg12084 rat. (a) β-Gal staining of a coronal section of a Tg12084 rat brain. Inset is a magnified region of the cortex depicting cortical layers I–VI. Scale bar = 1000 μM, inset = 500 μM. (b) EGFP positive cells expressing the transgene, (c) cells expressing the neuronal nuclear marker NeuN, and (d) merged image of (b) and (c) of layer III of the cortex. Scale bar (100 μM) in (b) also corresponds to (c) and (d). EGFP fluorescence (e–g), NeuN staining (h–j), and merged image (k–m) of the hippocampus (e, h, and k) and higher magnification of CA1 region (f, i, and l) identified by the solid boxes in (e), (h), and (k), and the CA3 region (g, j, and m), identified by the dashed boxes in (e), (h), and (k). Scale bar (500 μM) in (e) corresponds to (h) and (k). Scale bar (100 μM) in (f) corresponds to (g), (i), (j), (l), and (m)