Fig. 4

Spatial expression of RaPrnp-driven transgenes in adult rat brains. Fluorescence intensity in 9-month (a and b) and 1-year-old (c and d) rat brains. (a) Sagittal hemisphere of a Tg12084 rat brain demonstrates global EGFP fluorescence with peak fluorescence in the forebrain. (b) Sagittal hemisphere of a Tg12085 rat brain shows similar global EGFP signal, but the fluorescence is stronger in the brainstem, posterior, and midbrain compared with Tg12084 rats. Coronal serial slices through the brains of Tg12084 (c) and Tg12085 (d) rats show widespread EGFP signal. Top slices = midbrain. Middle slices = midbrain to forebrain. Bottom slices = forebrain. Brain stem (bs), caudoputamen (cp), cortex (ctx), corpus callosum (cc), cerebellum (cer), anterior forceps (fa), hippocampus (hipp), olfactory tuberde (ot), and thalamus (thm). Heat intensity maps of EGFP fluorescence depict low to high expression with a color gradient of blue, turquoise, green, orange, red, and white