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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Clusterin protects neurons against intracellular proteotoxicity

Fig. 3

Expression of CLU and TDP-43 in Drosophila. a Confocal microscopy images of imaginal eye discs of third instar larvae expressing human CLU in the photoreceptor cells; CLU (green), TOTO-3 (blue, detects nuclei). Left panel: Large white arrow indicates the morphogenetic furrow (scale bar 100 μm); white box represents the area shown at higher magnification in the large panel to the right. Right panel: Punctate staining of CLU (scale bar 10 μm); the inset white box shows a region with still higher magnification (arrowheads indicate CLU). b Non-reducing Western blots of Drosophila head homogenates, hemolymph (HL), and the positive control (human plasma CLU); expression of CLU indicated above the lanes. c Western blot of whole Drosophila head lysates, prepared from non-transgenic (Non-TG) Drosophila, or Drosophila expressing TDP-43, Htt-Q128 or Tau R406W; detection of XBP1-EGFP indicates activation of the UPR (β-actin was used as a loading control). d Western blot detecting CLU in hemolymph taken from Non-TG Drosophila, and those expressing CLU +/− TDP-43; equal amounts of hemolymph were loaded in each lane under non-reducing conditions. See also Additional file 1: Figure S6. e Confocal microscopy images of the central lobe of 10-day-old, whole adult Drosophila brains expressing CLU +/− TDP-43 in the motor neurons. Images in (i) and (iii) encompass the entire central lobe; the central region in each image contains neuronal process and axons. Images in (ii) and (iv) represent optical zooms of the areas indicated by the white boxes in (i) and (iii), respectively. Scale bars in (i) and (iii) represent 50 μm, while those in (ii) and (iv) represent 5 μm. CLU (green), white arrowheads in (iv) indicate axons. Results shown are representative of two or more independent experiments

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