Fig. 3

Methylation heatmap of pHGG tumors analyzed in this study. Primary and/or recurrent tumors from 16 pHGG samples from this study were analyzed with a large in-house dataset using 450 K methylation probes for clustering. In specific cases, tumor DNA from the primary only (HGG1, HGG3, HGG11) and recurrence only (HGG12) were available. Red bars within the colored group on the left represent the clustering of pHGG tumor samples within the known pHGG molecular group. Methylation groups are represented by colors: orange = H3K27M, green = H3G34R/V, blue = IDH1 mutant, purple = histone wildtype (WT). HGG case IDs for each tumor are indicated on the right. The label Prim/Rec indicates the clustering of both the primary and recurrence together. When the clustering location is different for the primary and recurrence tumor, the label is indicated as Prim (Primary) or Rec (Recurrence)