Fig. 9
From: Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions
Effect of TSP-1 depletion of C57ACM or supplementation to P301SACM on neuronal survival. To study the effect of TSP-1 on neuronal survival C57N and P301SN were cultured in (a, b) C57ACM or C57ACM depleted of TSP-1 or (c, d) P301SACM or P301SACM supplemented with TSP-1 for 8 days. a, c Neuronal cultures were fixed and immunolabelled with anti-β-III-tubulin antibody to determine neuronal number. The data represent a mean of three independent experiments. Each experiment consisted of three technical replicates (wells) in which at least three fields were analyzed. b *p < 0.05 for these comparisons: number of neurons in C57N + NB vs C57N + C57ACM; C57N + C57ACM vs C57N + C57ACM-TSP-1; C57N + NB vs C57N + C57ACM-TSP-1; P301SN + C57ACM vs P301SN + C57ACM-TSP-1. d *p < 0.05 for these comparisons: number of neurons in C57N + NB vs C57N + P301SACM; C57N + P301SACM vs C57N + P301SACM + TSP-1; P301SN + NB vs P301SN + P301SACM + TSP-1; P301SN + P301SACM vs P301SN + P301SACM + TSP-1. Results were evaluated with Tukey’s multiple comparisons. For experiments where TSP-1 was depleted ANOVA revealed significant interaction on genotype and culture condition (ACM) [F (2, 12) = 18.01; p = 0.0002], a significant effect for genotype [F (1, 12) = 22.87; p = 0.0004] and significant effect of culture type (ACM) [F (2, 12) = 32.75; p = 0.0001]. For experiments where TSP-1 were added, ANOVA revealed no difference between genotype and culture condition (ACM) [F (2, 12) = 2.524; p = 0.1217], a significant effect of the genotype [F (1, 12) = 9.39; p = 0.0098] and significant effect of culture type (ACM) [F (2, 12) = 32.75; p = 0.0001]